Image by Sarah Jane What is chlorophyll and what is photosynthesis?
Turn on the spectrophotometer to warm up the instrument and set the wavelength to nm by adjusting the wavelength control knob. While the spectrophotometer is warming up, your teacher may demonstrate how to prepare a chloroplast suspension from spinach leaves.
Set up an incubation area that includes a light, water flask, and test tube rack. Your teacher will provide you with two beakers, one containing unboiled chloroplasts.
Be sure to keep these on ice at all times. At the top rim, label the cuvettes 1,2,3,4, and 5, respectively. Using lens tissue, wipe the outside walls of each cuvette Remember: Using foil paper, cover the walls and bottom of cuvette 2.
Light should not be permitted inside cuvette 2 because it is a control for this experiment. Refer to Table 4. Do not add unboiled or boiled chloroplasts yet. To each cuvette, add 1 ml of phosphate buffer. Cover the top of cuvette 1 with Parafilm and invert to mix.
Cuvette 1 is the blank to be used to recalibrate the instrument between readings.
For each reading, make sure that the cuvettes are inserted into the sample holder so that they face the same way as in the previous reading. Obtain the unboiled chloroplast suspension, stir to mix, and transfer three drops to cuvette 2.
Immediately cover and mix cuvette 2. Replace cuvette 2 into the foil sleeve, and place it into the incubation test tube rack. Turn on the flood light. Take and record additional readings at 5,10,and 15 minutes.
Obtain the unboiled chloroplast suspension, mix, and transfer three drops to cuvette 3. Immediately cover and mix cuvette 3. Replace cuvette 3 into the incubation test tube rack.
Obtain the boiled chloroplast suspension, mix, and transfer three drops to cuvette 4. Immediately cover and mix cuvette 4.
Replace cuvette 4 into the incubation test tube rack. Cover and mix the contents of cuvette 5. Replace cuvette 5 into the incubation test tube rack.E) What gas does the plant provide for the child to use?
F) Will the plant continue to produce gas if the shade over the window is closed? G) According to the animation, what are the three reactants needed for a plant to.
Plant Pigments and Photosynthesis.
by Theresa Knapp Holtzclaw. Introduction. In photosynthesis, plant cells convert light energy into chemical energy that is stored in sugars and other organic compounds. Critical to the process is chlorophyll, the primary photosynthetic pigment in chloroplasts.
Photosynthesis: An Overview Lesson Objectives Explain the role of light and pigments in photosynthesis. Explain the role of electron carrier molecules in photosynthesis.
A. to protect a plant from losing water. B. to help plants absorb oxygen. C. to protect the plant cell.
This activity is a lab where students design an experiment to test the rate of photosynthesis. Students will analyze data,write a report using the scientific method, and apply results to .
The plant responded to the different colored light as follows: The rate of photosynthesis was the most successful in white light, and more successful in red and blue . Plant Pigments and Photosynthesis Introduction: In this laboratory you will separate plant pigments using chromatography.
You will also measure the rate of photosynthesis in isolated chloroplasts. The measurement technique involves the reduction of the dye DPIP. The transfer of electrons during the light-dependent reactions of photosynthesis reduces DPIP, changing it from blue to colorless.